爪蟾卵母细胞抽提物可诱导牛胎儿成纤维细胞发生部分重编程

doi:10.11843/j.issn.0366-6964.2015.09.009

Abstract

Abstract:

In order to reprogram the Japanese Black cattle fetal fibroblasts(JBCFF)，the Xenopus laevis oocyte extracts was used to incubation with the cells in present study.After 24 hours of culture in medium，there was no significant difference in acetylation of histone H3K9 between the JBCFF treated with extracts and the cells untreated by immunofluorescence assay.The well-defined colony structures of JBCFF treated with extracts were formed with the continuous culture for 5-6 d and the alkaline phosphatase staining of the cells colony was positive.Similarly，the OCT4 protein was detected in the cells colony.Additionally，Oct4 and Nanog mRNA expression，two pluripotency marker genes，were detected in those cells by RT-PCR，but Sox2 gene expression was not detected.Furthermore，qPCR results showed that the expression levels of Oct4 and Nanog genes in colony cells increased with days of culture(4，5，6 d ) after extracts treatment.In conclusion，the Xenopus laevis oocyte extracts could induce the partial reprogramming of the JBCFF into a low differentiated state.

CLC Number:

S823.2

DU Wei-hua，FAN Zong-xing，WANG Hao-yu，HAO Hai-sheng，LIU Yan，ZHAO Xue-ming，QIN Tong，ZHU Hua-bin. Reprogramming of Japanese Black Cattle Fetal Fibroblasts Treated with Xenopus laevis Oocytes Extracts[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1549-1556.

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Reprogramming of Japanese Black Cattle Fetal Fibroblasts Treated with Xenopus laevis Oocytes Extracts

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